Rapid Methods for Determining Decarboxylase Activity: Ornithine and Lysine Decarboxylases
نویسندگان
چکیده
منابع مشابه
Rapid methods for determining decarboxylase activity: ornithine and lysine decarboxylases.
A rapid biochemical method for the determination of ornithine and lysine decarboxylase (EC 4.1.1.18) activity has been developed for use in the routine clinical laboratory. It is based on the detection of the amine end product produced in response to the single key amino acid added to a synthetic medium. A modified ninhydrin reagent is used to detect the amine after a chloroform extraction. Thi...
متن کاملRapid methods for determining decarboxylase activity: arginine decarboxylase.
A rapid biochemical method for the determination of arginine decarboxylase (EC 4.1.1.19) activity has been developed for use in the routine clinical microbiology laboratory and correlated with similar procedures for ornithine and lysine decarboxylase (EC 4.1.1.18) systems. It is based on the detection of agmatine, the amine end product formed during growth on a synthetic medium containing argin...
متن کاملA rapid method for determining decarboxylase and dihydrolase activity.
A total of 764 fresh clinical isolates were used to test a rapid method for determining lysine, arginine, and ornithine decarboxylase activity as well as arginine dihydrolase activity. The conventional Møller decarboxylase broth was tested in parallel with the rapid method on 234 Enterobacteriaceae and 140 non-fermentative Gram-negative rods. The 0.3% agar method was tested in parallel on 245 E...
متن کاملRapid and regulated degradation of ornithine decarboxylase.
Ornithine decarboxylase (ODC; EC 4.1.1.17), the first and key enzyme in polyamine biosynthesis (Figure 1), turns over extremely rapidly and is one of the most highly regulated enzymes known [1-3]. It is induced by many kinds of growth stimuli, and its suppression by specific inhibitors or mutation inhibits cellular growth and transformation [4,5]. A recent study showed that overexpression of OD...
متن کاملUse of gas chromatography for detecting ornithine and lysine decarboxylase activity in bacteria.
A gas-liquid chromatography (GLC) procedure for the detection of L-ornithine and L-lysine decarboxylase (EC 4.1.1.17 and EC 4.1.1.18, respectively) activities of bacteria was developed and evaluated against Møller's method, a conventional biochemical test. Cultures were incubated for 2 to 4 h in a simple growth medium and tested by GLC for putrescine and cadaverine, the direct decarboxylation p...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Applied Microbiology
سال: 1971
ISSN: 0003-6919
DOI: 10.1128/aem.22.3.344-349.1971